China Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit emepụta na suppliers |Yinye

Akwụkwọ akụkọ Coronavirus (SARS-CoV-2) Ngwa PCR Real-Time Multiplex

Akwụkwọ akụkọ Coronavirus (SARS-CoV-2) Ihe osise PCR adịgboroja ezigbo oge

Nkọwa dị mkpirikpi:

REF 500190 Nkọwapụta 96 Nnwale/igbe

Ụkpụrụ nchọpụta PCR Ụdị Ọkpụkpụ imi / Nasopharyngeal swab

Nkọwa ngwaahịa

Mkpado ngwaahịa

Ngwa PCR a dị njikere nke ukwuu dị n'ụdị lyophilized (usoro ihicha oyi) maka nchekwa ogologo oge.Enwere ike ibufe ngwa ahụ ma chekwaa ya na ụlọ okpomọkụ ma kwụsie ike maka otu afọ.Igwe premix ọ bụla nwere reagents niile achọrọ maka nkwalite PCR, gụnyere Reverse-transcriptase, Taq polymerase, primers, probes, na dNTPs substrates.Ọ chọrọ naanị tinye 13ul distilled mmiri na 5ul amịpụtara RNA template, mgbe ahụ enwere ike ịgba ya ma gbasaa na ngwa PCR.

Igwe qPCR kwesịrị izuteIhe ndị a chọrọ:

1. dabara 8 warara PCR tube olu 0.2 ml
2. Nwee ọwa nchọpụta ihe karịrị anọ:

Ọwa	Mmalite (nm)	Mgbapụta (nm)	Ejiji agbachagoro tupu oge eruo
1.	470	525	FAM, SYBR Green I
2	523	564	VIC, HEX, TET, JOE
3.	571	621	ROX, TEXAS-RED
4	630	670	CY5

PCR-ikpo okwu:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR Detection System, Stratagene Mx3000P, Mx3005P

Ihe isi ike nke oyi yinye njem nkeNchọpụta nucleic acid novel Coronavirus reagent

Mgbe a na-ebufe reagents nchọpụta nucleic acid a na-ahụkarị n'ebe dị anya, a chọrọ nchekwa (-20± 5) ℃ oyiri yinye na njem iji hụ na bioactive nke enzyme na reagents ka na-arụ ọrụ.Iji jide n'aka na okpomọkụ ahụ ruru ọkọlọtọ, a chọrọ ọtụtụ kilogram nke ice akọrọ maka igbe ọ bụla nke reagent nucleic acid test ọbụna ihe na-erughị 50g, mana ọ nwere ike ịdịru naanị ụbọchị abụọ ma ọ bụ atọ.N'ihe banyere omume ụlọ ọrụ, n'ezie arọ nke reagents nyere site emepụta bụ ihe na-erughị 10% (ma ọ bụ nnọọ ihe na-erughị nke a uru) nke akpa.Ọtụtụ n'ime arọ ahụ na-abịa site na ice akọrọ, ngwugwu ice na igbe ụfụfụ, ya mere ọnụ ahịa njem dị oke elu.

Na Machị 2020, COVID-19 malitere ibipụta n'ọtụtụ mba ofesi, na ọchịchọ maka nchọpụta Nucleic acid Novel Coronavirus reagent mụbara nke ukwuu.N'agbanyeghị ọnụ ahịa dị elu nke mbupụ reagents na agbụ oyi, ọtụtụ ndị na-emepụta ihe ka nwere ike ịnakwere ya n'ihi nnukwu ego na nnukwu uru.

Otú ọ dị, na mmelite nke mba mbupụ atumatu maka mgbochi ọrịa na ngwaahịa, nakwa dị ka upgrading nke mba akara n'elu eruba nke ndị mmadụ na lọjistik, e nwere ndọtị na ejighị n'aka na njem oge nke reagents, nke mere ka a ma ama ngwaahịa nsogbu kpatara. site na njem njem.Ogologo oge njem njem (oge mbufe ihe dị ka ọkara ọnwa na-adịkarị) na-eduga na ọdịda ngwaahịa ugboro ugboro mgbe ngwaahịa ahụ ruru onye ahịa ahụ.Nke a agbaghawo ọtụtụ ụlọ ọrụ nucleic acid reagents na-ebupụ.

Teknụzụ Lyophilized maka PCR reagent nyeere ndị njem nkeNchọpụta nucleic acid novel Coronavirus zuru ụwa ọnụ

Enwere ike iburu PCR reagents lyophilized ma chekwaa ya na ụlọ okpomọkụ, nke nwere ike ọ bụghị naanị ibelata ọnụ ahịa njem, kamakwa zere nsogbu dị mma nke usoro njem na-akpata.Ya mere, lyophilizing reagent bụ ụzọ kachasị mma isi dozie nsogbu nke njem mbupụ.

Lyophilization gụnyere ịsacha ihe ngwọta n'ime ọnọdụ siri ike, wee sublimate ma kewaa vapo mmiri n'okpuru ọnọdụ agụụ.Solute a mịrị amị na-anọgide n'ime akpa ahụ nwere otu ihe mejupụtara na ọrụ.E jiri ya tụnyere reagents mmiri mmiri a na-ahụkarị, ihe mejupụtara lyophilized Novel Coronavirus nucleic acid reagent nke Liming Bio mepụtara nwere njirimara ndị a:

Nkwụsi ike okpomọkụ siri ike:ọ nwere ike iji ọgwụgwọ guzoro na 56 ℃ maka ụbọchị 60, na morphology na arụmọrụ nke reagent na-agbanwe agbanwe.
Nchekwa okpomọkụ na njem ụgbọ njem nkịtị:ọ dịghị mkpa maka yinye oyi, ọ dịghị mkpa ịchekwa ya na obere okpomọkụ tupu ịmeghe ya, hapụ ebe nchekwa oyi zuru oke.
Dị njikere iji:lyophilizing nke ihe niile, ọ dịghị mkpa maka nhazi usoro, na-ezere mfu nke ihe ndị nwere nnukwu viscosity dị ka enzyme.
Ebumnuche Multiplex na otu tube:ebumnuche nchọpụta ahụ na-ekpuchi akwụkwọ akụkọ coronavirus ORF1ab gene, N gene, S gene iji zere mkpụrụ ndụ ihe nketa nje.Iji wedata adịghị mma ụgha, a na-eji mkpụrụ ndụ RNase P mmadụ mee ihe dị ka njikwa ime, iji gboo mkpa ụlọ ọgwụ maka njikwa mma nlele.