Akwụkwọ akụkọ Coronavirus (2019-nCoV) Ngwa nchọpụta Ribonucleic Acid (PCR adịla oge - Fluorescent Probe Assay)
Eburu n'obiJiri
Ezubere ngwaahịa a maka nchọpụta qualitative coronavirus (SARS-CoV-2) n'omume iku ume.Enwere ike iji nsonaazụ nchọpụta nyere aka nyocha ụlọ ọgwụ nke Ọrịa Coronavirus 2019 (COVID-19).
Ngwa
Ihe nchekwa PCR 2019-nCoV - ule 96
Ngwakọta Enzyme PCR - Nnwale 96
Njikwa mma 2019-nCoV - ule 48
Njikwa adịghị mma 2019-nCoV - ule 48
Njikwa ime 2019-nCoV - ule 96
Ntinye ngwugwu - 1 oyiri
NgwaahịaMechanism
Ngwaahịa a nwere ụzọ anọ nke PCR primers, nyocha fluorescent anọ, RNA reverse transcriptase, DNA polymerase, dNTP, magnesium ion, na kemịkalụ ndị ọzọ maka nchọpụta nje RNA.Ọ na-elekwasị anya na RNA-based RNA polymerase (RdRp), gene Nucleocapsid (N), na Envelop (E) gene nke 2019-nCoV n'otu oge n'otu tube ule.Usoro nnwale na-aga n'ime usoro atọ ndị a: 1) tụgharịa sụgharịa viral RNA ka ọ bụrụ DNA viral.2) Gbanwee DNA viral ka ọ bụrụ nha nha.3) Kpesa ọnụ ọgụgụ nke amplicon DNA site na ngwakọ nyocha.E wezụga nke ahụ, ngwaahịa a tinyekwara njikwa ime na njikwa mpụga iji nyochaa arụmọrụ ya n'oge njem na nchekwa.
